digestive conditions (stomach pain, bad or slow digestion and intestinal gas | TRAMIL

	Allium sativum (In territories with significant traditional TRAMIL use) Barbados : garlic Dominican Republic : ajo Guatemala : ajo Haiti : lay Saint Lucia : lay Guadeloupe : ail Guadeloupe : lay Significant uses found by the TRAMIL surveys bulb, decoction, taken orally^1-6^,74,75	Recommandations Preparation and posology References	Use for “bad blood” is a traditional cultural use of our communities and is not classified in the TRAMIL Program. According to published and other information: Use for skin conditions, pruritus, candidiasis, gastric bloating, nausea, flatulence, and intestinal parasites is classified as REC, based on significant traditional use documented in the TRAMIL surveys and published scientific information. Use for toothaches, earaches, and fever is classified as REC, based on significant traditional uses (OMS/WHO)⁷ documented by TRAMIL surveys. The bulb can cause reactions of hypersensitivity. For topical application, strict hygiene measures should be observed in order to avoid contamination or additional infection. Due to the possibility that an earache could signal a middle or inner ear infection, immediate medical evaluation is recommended. Do not use if there are secretions from the ear and/or possible perforation of the eardrum. If the patient’s condition worsens or if the earache persists for more than 2 days, seek medical attention. Contraindicated for use in hyperthyroidism⁸. The extracts from garlic interact with anticoagulant, antihypertensive and antidiabetic treatments.		The bulb of Allium sativum is widely used for human consumption. The garlic bulb for medicinal purposes should be taken together with meals, so as to prevent gastro-intestinal problems⁷¹. For fever, gastric bloating, nausea, flatulence and intestinal parasites: Prepare a decoction with 2 to 5 grams of fresh garlic⁷¹ (1 to 2.5 cloves) or 2 to 4 grams of dried garlic⁷¹ in 1 cup of water (250 mL), boil for at least 10 minutes in a covered pot. Cool and drink 1 cup 3 times a day. For other forms of administration of garlic bulb, the daily consumption doses recommended by OMS⁷¹ are: for oil: 2 to 5 mg; for extracts, 300 to 1000 mg (as solid material). Other type of pharmaceutical preparations should meet alliine values of 4-12 mg or allicine values of 2-5 mg⁷¹. For treatment of skin conditions, pruritus, candidiasis, toothache, and earache: 2 to 5 g (1 to 2.5 cloves) of peeled fresh garlic; wash thoroughly and crush, then apply to the affected area twice a day.	1 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 2 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 3 GIRON L, 1988 Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala. 4 WENIGER B, 1987-88 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 5 JEAN-PIERRE L, 1988 TRAMIL survey. St Lucia national herbarium, Castries, St Lucia. 6 FAUJOUR A, MURREY D, CHELTENHAM-CORBIN B, CARRINGTON S, 2003 TRAMIL survey. enda-caribbean, IICA & UAG, Saint Thomas, Barbados. 7 WHO, 1991 Pautas para la evaluación de medicamentos herbarios WHO/TRM/91.4 (original inglés). Programa de Medicina Tradicional, OMS, Ginebra, Suiza. 8 ARTECHI A (Ed.), 1998 Fitoterapia Vademécum de prescripción Plantas Medicinales. Barcelona, España: Masson. p63. 9 SENDL A, ELBL G, STEINKE B, REDL K, BREU W, WAGNER H, 1992 Comparative pharmacological investigations of Allium ursinum and Allium sativum. Planta Med 58:11-17. 10 CALVEY EM, ROACH JAG, BLOCK E, 1994 Supercritical fluid chromatography of garlic (Allium sativum) extracts with mass spectrometric identification of allicin. J Chromatogr Sci 32:393-396. 11 BLOCK E, AHMAD S, CATALFAMO JL, JAIN MK, APITZ-CASTRO R, 1986 Antithrombotic organosulfur compounds from garlic: structural, mechanistic, and synthetic studies. J Amer Chem Soc 108:227045-227055. 12 MOCHIZUKI E, YAMAMOTO T, KOMIYAMA Y, NAKAZAWA H, 1998 Identification of Allium products using flame photometric detection gas chromatography and distribution patterns of volatile sulfur compounds. J Agr Food Chem 46:125170-125176. 13 UEDA Y, SAKAGUCHI M, HIRAYAMA K, MIYAJIMA R, KIMIZUKA A, 1990 Characteristic flavor constituents in water extract of garlic. Agr Biol Chem 54:1163-1169. 14 KOCH HP, JAGER W, 1989 Garlic allicin release from fresh and dried garlic and products thereof. Dtsch Apoth Ztg 129:6273-6276. 15 LAWSON LD, WANG ZYJ, HUGHES BG, 1991 Identification and hplc quantitation of the sulfides and dialk(en)yl thiosulfinates in commercial garlic products. Planta Med 57:363-370. 16 OHSUMI C, HAYASHI T, KUBOTA K, KOBAYASHI A, 1993 Volatile flavor compounds formed in an interspecific hybrid between onion and garlic. J Agr Food Chem 41:101808-101810. 17 LAWSON LD, WOOD SG, HUGHES BG, 1991 hplc analysis of allicin and other thiosulfinates in garlic clove homogenates. Planta Med 57:3263-3270. 18 MUTSCH-ECKNER M, ERDELMEIER CAJ, STICHER O, REUTER HD, 1993 A novel amino acid glycoside and three amino acids from Allium sativum. J Nat Prod 56:6864-6869. 19 INAGAKI M, HARADA Y, YAMADA K, ISOBE R, HIGUCHI R, MATSUURA H, ITAKURA Y, 1998 Isolation and structure determination of cerebrosides from garlic, the bulbs of Allium sativum L. Chem Pharm Bull 46:71153-71156. 20 AL-NAGDY SA, ABDEL-RAHMAN MO, HEIBA HI, 1988 Evidence for some prostaglandins in Allium sativum extracts. Phytother Res 2:4196-4197. 21 ROLLERI F, 1943 Occurrence of nicotinic acid and nicotinamide in curative plants. Arch Pharm (Weinheim) 281:118. 22 RAKHIMBAEV IR, OL'SHANSKAYA RV, 1981 Preliminary identification of natural gibberellins of garlic. Izv Akad Nauk Kaz Ssr Ser Biol 1981:217-222. 23 KOCH HP, JAGER W, GROH U, HOVIE JE, PLANK G, SEDLAK U, PRAZNIK W, 1993 Carbohydrates from garlic bulbs (Allium sativum L.) As inhibitors of adenosine deaminase enzyme activity. Phytother Res 7(5):387-389. 24 BAUMGARTNER S, DAX TG, PRAZNIK W, FALK H, 2000 Characterisation of the high-molecular weight fructan isolated from garlic (Allium sativum L.). Carbohydr Res 328:2177-2183. 25 PENG JP, CHEN H, QIAO YQ, MA LR, NARUI T, SUZUKI H, OKUYAMA T, KOBAYASHI H, 1996 Two new steroidal saponins from Allium sativum and their inhibitory effects on blood coagulability. Yao Hsueh Hsueh Pao 31:8607-8612. 26 MATSUURA H, USHIROGUCHI T, ITAKURA Y, FUWA T, 1989 Further studies on steroidal glycosides from bulbs, roots and leaves of Allium sativum L. Chem Pharm Bull 37:102741-102743. 27 DUKE JA, ATCHLEY AA, 1986 Handbook of proximate analysis tables of higher plants. Boca Ratón, USA: CRC Press. p13. 28 DAS I, KHAN N, SOORANA S, 1995 Potent activation of nitric oxide synthase by garlic: a basic for therapeutic application. J Med Res Opin 5(13):257-263. 29 KABELIK J, 1970 Antimicrobial properties of garlic. Pharmazie 25(4):266-270. 30 ADETUMBI M, JAVOR G, LAN B, 1986 Allium sativum (garlic) inhibits lipid synthesis by Candida albicans. Antimicrob Agents Chemother30(3):499-501. 31 TSAI Y, COLE LL, DAVIS LE, LOCKWOOD SJ, SIMMONS V, WILD GC, 1985 Antiviral properties of garlic: in vitro effects onInfluenza b, Herpes simplex and Coxsackie viruses. Planta Med 51(5):460-461. 32 SHARMA V, SETHI M, KUMAR A, RAROTRA JR, 1977 Antibacterial property of Allium sativum in vivo & in vitro studies. Indian J Exp Biol15:466. 33 FROMTLING R, BULMER G, 1978 In vitro effect of aqueous extract of garlic on the growth and viability of Cryptococcus neoformans. Mycology 70:397-400. 34 GRAHAM H, GRAHAM E, 1987 Inhibition of Aspergillus parasiticus growth andtoxin productionby garlic. Journal Food Safety 8:101-108. 35 MOLINA NM, 1992 Actividad antimicrobiana de extractos de Allium sativum. Evaluación preclínica, farmacológica y toxicológica (Tesis de graduación). Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. p287. 36 EL-TANBOULY ND, ABDEL-MEGID RM, 1994 HPLC determination of allicin in garlic and garlic products and their antimicrobial activity. Zagazig J Pharm Sci 3(3A):120-124. 37 CACERES A, GIRON L, ALVARADO S, TORRES M, 1987 Screening of antimicrobial activity of plants popularly used in Guatemala for the treatment of dermatomucosal diseases. J Ethnopharmacol 20(3):223-237. 38 KIUCHI ,F, NAKAMURA N, MIYASHITA N, NISHIZAWA S, TSUDA Y, KONDO K, 1989 Nematocidal activity of some anthelmintics, traditional medicines, and spices by a new assay method using larvae of Toxocara canis. Shoyakugaku Zasshi 43(4):279. 39 CHOWDHURY A, AHSAN M, ISLAM SK, AHMED ZU, 1991 Efficacy of aqueous extract of garlic and allicin in experimental shiguellosis in rabbits. Indian J Med Res93(1):33-36. 40 STANDEN O, 1953 Experimental chemotherapy of oxyuriasis. Brit Med JII:757-758. 41 TAKASUGI N, KOTOO K, FUWA T, SAITO H, 1984 Effect of garlic on mice exposed to various stresses. Oyo Yakuri Pharmacolo 28(6):991-1002. 42 PRASAD DN, BHATTACHARYA SK, DAS PK, 1966 A study of antinflammatory activity of some indigenous drugs in albino rats. Indian J Med Res 54:582. 43 BHAKUNI D, DHAR ML, DHAR MM, DHAWAN B, MEHROTRA B, 1971 Screening of Indian plants for biological activity. Part III. Indian J Exp Biol 9:91. 44 AUGUSTI K, MATHEW P, 1973 Effect on long-term feeding of the aqueous extracts of onion and garlic on normal rats. Ind J Exper Biol11(3):239-240. 45 HIKINO H, TOHKIN M, KISO Y, NAMIKI T, NISHIMURA S, TAKEYAMA K, 1986 Antihepatotoxic actions of Allium sativum bulbs. Planta Med 52(3):163-168. 46 SHARAF A, 1969 Food plant as a possible factor in fertility control. Qual Plant MaterVeg17:153. 47 PRASAD G, SHARMA V, KUMAR A, 1982 Efficacy of garlic (Allium sativum L.) therapy against experimental dematophytosis in rabbits. Indian J Med Res75:465-467. 48 TUTAKNE M, BHARDWAJ J, SATYANARAYANAN G, SETHI Y, 1983 Sporotrichosis treated with garlic juice. Indian J Dermatology 28:40-47. 49 DELAHA E, GARAGUSI V, 1985 Inhibition of mycobacteria by garlic extract (Allium sativum ). Antimicrob Agents Chemother27(4):485-486. 50 PETKOV V, 1966 Pharmacological and clinical study of garlic. Dtsch Apoth Ztg 106(51):1861-1867. 51 FOUSHEE D, RUFFIN J, BANERJEE U, 1982 Garlic as a natural agent for the treatment of hypertension: a preliminary report. Cytobios34(135-136):145-152. 52 PARIS R, MOYSE H, 1981 Précis de Matière Médicale, Tome II, 2^è éd. révisée. Paris, France: Ed. Masson. p61-62. 53 RIBEIRO R, BARROS F, MARGARIDA M, MELO R, MUNIZ C, CHIEIA S, WANDERLEY M, GOMES C, TROLIN G, 1988. Acute diuretic effects in conscious rats produced by some medicinal plants used in the state of Sao Paulo, Brasil. J Ethnopharmacol 24(1):19-29. 54 SPADA C, HOSHIKAWA-FUJIMURA AY, 2001 Perspectivas do emprego do Allium sativum (alho) em estudos epidemiológicos da patologia cardiovascular (conferencia). Caxambu, Brazil: Congresso da VII Reunião Anual da Federação de Sociedades de Biologia Experimental. 55 CHUTANI SK, BORDIA A, 1981 The effect of fried versus raw garlic on fibrinolytic activity in man. Therosclerosis 38:417-421. 56 DAMRAU F, FERGUSON E, 1949 The modus vivendi of carminative. The therapeutic value of garlic in functional gastrointestinal disorders. Rev Med J2:757-758. 57 YU WC, BLOT WJ, CHANG YS, ERSHOW A, ZT YANG, AN Q, FRAUMENI JR JF, WANG TG, 1989 Allium vegetables and reduced risk of stomach cancer. J Natl Cancer Inst 81(2):162-164. 58 FERNANDEZ EJ, 1995 Efecto de dos formulaciones hidroalcohólicas de Allium sativum en el tratamiento de pacientes con dermatofitosis (Tesis de especialidad en farmacología). Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 59 ALONSO JR, 1998 Tratado de fitomedicina. Bases clínicas y farmacológicas. Buenos Aires, Argentina: Ed. ISIS S.R.L. p208. 60 DURAFFOURD C, D'HERVICOURT L, LAPRAZ JC, 1994 Cahiers de phytothérapie clinique. Paris, France: Revue de la Societé Française de Phytothérapie et Aromathérapie. 61 CHAUHAN L, GARG J, BEDI H, GUPT R, BOMB B, AGARWAL M, 1982 Effect of onion, garlic and clofibrate on coagulation and fibrinolytic activity of blood in cholesterol fed rabbits. Indian Med J 76(10):126-127. 62 KANEZAWA A, NAKAGAWA S, SUMIYOSHI H, MASAMOTO K, HARADA H, NAKAGAMI S, DATE S, YOKOTA A, NISHIKAWA M, FUWA T, 1984 General toxicity tests of garlic extract preparation contained vitamins (Kyoleopin). Pharmacometrics 27(5):909-929. 63 SUMIYOSHI H, KANEZAWA A, MASAMOTO K, HARADA H, NAKAGAMI S, YOKOTA A, NISHIKAWA M, NAKAGAWA S, 1984 Chronic toxicity test of garlic extract in rats. J Toxicol Sci 9:61-75. 64 AL-BEKAIRI AM, SHAH AH, QURESHI S, 1990 Effect ofAllium sativum on epididymal spermatozoa, estradiol-treated mice and general toxicity. J Ethnopharmacol 29(2):117-125. 65 HUH K, PARK JM, LEE S-IL, 1985 Effect of garlic on the therapeutic glutathione S-transferase and glutathione peroxidase activity in rat. Arch Pharm Res 8(4):197-203. 66 GARNIER G, BEZANGER-BEAUQUESNE L, 1961 Ressources médicinales de la flore française. Paris, France: Ed. Vigot Frères. 67 BOJS G, SVENSSON A, 1988 Contact allergy to garlic used for wound healing. Contact Derm18(3):179-181. 68 PAPAGEORGION C, CORBET JP, BRANDAO FM, PECEGUEIRO M, BENEZIA C, 1983. Allergic contact dermatitis to garlic (Allium sativum) identification of the allergens the role of mono-di and trisulfides present in Garlic. A comparative study in man and animal. Arch Dermatol Res 275(4):229-234. 69 CAPORASO N, SMITH S, ENG R, 1983 Antifungal activity in human urine and serum after ingestions of garlic. Antimicob Agents Chemother23(5):700-702. 70 SITPRIJA S, PLENGVIDHYA C, KANGKAYA V, BHUVAPANICH S, TUNKAYOON M, 1987. Garlic and diabetes mellitus phase III clinical trial. J Med Assoc 70(2):223-227. 71 WHO, 2004 Allium sativum. WHO monographs on selected medicinal plants. Volume 1. Bulbis Alii Sativi. Ginebra, Suiza, Oct.28,2004. URL: http://www.who.int/medicines/library/trm/medicinalplants/monograph_volum... 72 MARTINEZ MJ, MOREJON Z, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2005 Irritabilidad dérmica primaria de bulbo fresco machacado de Allium sativum L. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Medicina “Dr. Salvador Allende”, Cerro, C. Habana, Cuba. 73 MARTINEZ MJ, MOREJON Z, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2005 Clases tóxicas agudas (CTA) de una decocción de bulbo de Allium sativum L. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 74 BOULOGNE Isabelle, 2008 Enquête TRAMIL, Les Saintes, UAG, Guadeloupe (FWI). 75 LONGUEFOSSE JL, NOSSIN E, 1990-95 Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique
	Petiveria alliacea (In territories with significant traditional TRAMIL use) Colombia : anamú Dominica : koujourouk Dominican Republic : anamú Guatemala : apacín Honduras : ipacina Haiti : ave Panama : anamú Significant uses found by the TRAMIL surveys leaf, decoction, orally¹	Recommandations Preparation and posology References	According to published and other information: Use for digestive conditions (stomach pain, bad or slow digestion and intestinal gas) toothache, muscle pain, skin diseases, rheumatism and common cold is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies. Should there be a notable worsening of the patient’s condition, or should stomach pain persist for more than 3 days, seek medical attention. Use for headache, flatulence, flu and nasal congestion (sinusitis) is classified as REC, based on the significant traditional use (OMS/WHO)¹¹ documented in the TRAMIL surveys. For topical application, strict hygiene measures should be observed in order to avoid contamination or additional infection. Considering the risks of documented interactions with insulin or oral hypoglycemiants, the decoction of the leaf and stem should not be ingested by patients taking or using these medicines due to the risk of magnifying their effects. The root and the stem can cause reactions of hypersensitivity. Not for use during lactation or by children under 12 years old. Not for use during pregnancy because it may be abortifacient.		For digestive conditions and common cold: Prepare a decoction with 30 grams (3 spoonfuls) of ground leaf in 1 liter of water (4 cups). Boil for at least 10 minutes in a covered pot. Filter, allow to cool and drink 2-3 cups a day³⁷. For rheumatism: Prepare a decoction with 30 grams (3 spoonfuls) of ground leaf and root in 1 liter of water (4 cups) for at least 10 minutes in a covered pot. Filter, leave to cool down and drink 2-3 cups a day³⁷. For nasal congestion (sinusitis): Prepare a fine powder of the root and stem from dried and sieved material, inhale 0.2 to 0.5 grams through each nostril, 2 times a day³⁸. For headache, toothache, muscle pain, flatulence, flu and skin diseases: There is no available information establishing a means of preparation and dosage other than that referred to by traditional use. Any medicinal preparation must be preserved cold and used within the 24 hours.	1 GIRON L, 1988 Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala. 2 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 3 WENIGER B, 1987-88 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 4 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (región Este). Dep. de Botánica, Jardín Botánico Nacional, Santo Domingo, Rep. Dominicana. 5 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 6 LAGOS-WITTE S, Tinoco R, Merlo V, 1996 Encuesta complementaria TRAMIL. Laboratorio de Histología Vegetal y Etnobotánica, Dep. de Biología, Universidad Nacional Autónoma de Honduras UNAH, Tegucigalpa, Honduras. 7 GOMEZ H, GAITAN R, DIAZ F, 2003 Encuesta TRAMIL (Norte del departamento de Bolívar). Grupo de Productos Naturales, Facultad de Ciencias Químicas y Farmacéuticas. Universidad de Cartagena, Cartagena de Indias, Colombia. 8 SOLIS P, CORREA M, GUPTA M, 1995 Encuesta TRAMIL (Comunidades afro-caribeñas). Centro de Investigaciones Farmacognósticas de la Flora Panameña CIFLORPAN, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 9 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 10 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (Zambrana, Cotuí). Dep. de Botánica, Jardin Botánico Nacional, Santo Domingo, Rep. Dominicana. 11 WHO, 1991 Pautas para la evaluación de medicamentos herbarios WHO/TRM/91.4 (original inglés). Programa de Medicina Tradicional, OMS, Ginebra, Suiza. 12 WENIGER B, SAVARY H, DAGUIHL R, 1984 Tri phytochimique de plantes de la liste TRAMIL. Laboratoire de chimie des substances naturelles, Faculté de Médecine et de Pharmacie, Université d'Etat d'Haïti, Port au Prince, Haïti. 13 HEGNAUER R, 1973 Chemotaxonomy der Pflanzen. Basel, Schweiz: Birkhauser Verlag. 6:882. 14 SEGELMAN F, SEGELMAN A, 1975 Constituents of Petiveria alliacea. Lloydia 38(6):537. 15 DE SOUSA JR, DEMUNER AJ, PINHEIRO JA, BREITMAIER E, CASSELS BK, 1990 Dibenzyl trisulphide and trans-N-methyl-4-methoxyproline fromPetiveria alliacea. Phytochemistry 29(11):3653-3655. 16 FURONES JA, MORON F, PINEDO Z, 1996 Ausencia de la acción analgésica de la Petiveria alliacea (anamu) en ratones. Rev Cubana Planta Med 1(1):16-18. 17 DEL CARMEN RIVAS C, JIMENEZ M, AYALA L, CARILLO C, CABRERA Y, 1988 Actividad anti-inflamatoria y analgésica dePetiveria alliaceae. Informe TRAMIL. Centro de Investigación y Desarrollo de Medicamentos (CIDEM), La Habana, Cuba. 18 FURONES JA, MORON F, PINEDO Z, 1996 Ausencia de actividad antiinflamatoria del extracto acuoso liofilizado de Petiveria alliacea (anamú) en ratas. Informe TRAMIL. Rev Cubana Planta Med 1(2):34-37. 19 Martinez MJ, Betancourt J, Lopez M, MorejOn Z, Fuentes V, MORON F, PINEDO Z, Boucourt E, 2001 Actividad antimicrobiana y sobre varias preparaciones de músculo liso, in vitro, de la decocción liofilizada de hoja de Petiveria alliacea.Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 20 GarcIa GM, Coto MT, GonzAlez CS, Pazos L, 1995 Velocidad del tránsito intestinal en ratones, del extracto acuoso de hoja fresca de Petiveria alliacea. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBI, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 21 ECHEVARRIA A, TORRES D, 2001 Efecto de un extracto de Petiveria alliacea Lin sobre el crecimiento de Giardia lamblia in vitro. Rev Cubana Med Mil 30(3):161-165. 22 CACERES A, GIRON LM, ALVARADO SR, TORRES MF, 1987 Screening of antimicrobial activity of plants popularly used in Guatemala for the treatment of dermatomucosal diseases. J Ethnopharm20(3):223-237. 23 CACERES A, LOPEZ BR, GIRON MA, LOGEMANN H, 1991 Plants used in Guatemala for the treatment of dermatophytic infections. 1. Screening for the antimicotic activity of 44 plant extracts. J Ethnopharm 31(3):263-276. 24 SAUVAIN M, 1989 Etude de plantes antiparasitaires du plateau des Guyanes en Amazonie: antipaludiques et antileishmaniens (Thèse de Doctorat). Université Paris-Sud, Paris, France. 25 RUFFA MJ, PERUSINA M, ALFONSO V, WAGNER ML, SURIANO M, VICENTE C, CAMPOS R, CAVALLARO L, 2002 Antiviral activity of Petiveria alliacea against the bovine viral diarrhea virus. Chemotherapy 48(3):144-147. 26 CACERES A, JAUREGUI E, HERRERA D, LOGEMANN H, 1991 Plants used in Guatemala for the treatment of dermatomucosal infections. 1: Screening of 38 plant extracts for anticandidal activity. J Ethnopharm 33(3):277-283. 27 LORES RI, PUJOL MC, 1990 Petiveria alliacea L. (anamu). Study of the hypoglycemic effect. Med Interne 28(4):347-352. 28 GERMANO DH, CALDEIRA TT, MAZELLA AA, SERTIE JA, BACCHI EM, 1993 Topical anti-inflammatory activity and toxicity of Petiveria alliacea. Fitoterapia 64(5):459-467. 29 FERRAZ MB, PEREIRA RB, IWATA NM, ATRA E, 1991 Tipi. A popular analgesic tea. A double blind cross-over trial in osteoarthritis. Clin Exp Rheumatol 9(2):205-206. 30 LIMA TCM, MORATO GS, TAKAHASHI RN, 1991 Evaluation of antinociceptive effect of Petiveria alliacea (guiné) in animals. Mem Inst Oswaldo Cruz 86(suppl.2):153-158. 31 GarcIa GM, Coto MT, GonzAlez CS, Pazos L, 1996 Toxicidad sub-crónica en ratones, del extracto acuoso de hojas frescas de Petiveria alliacea. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBI, Universidad de Costa Rica, San Pedro, Costa Rica. 32 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, MORON F, 2001 Toxicidad aguda (DL₅₀) oral de la decocción de hojas jóvenes frescas de Petiveria alliaceaL.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende. Ciudad de La Habana, Cuba. 33 GUERRA MO, OLIVEIRA AB, MAIA JGS, PETERS VM, 1989 Alteraçäo do desenvolvimento embrionário de ratos após tratamento com extratos aquosos de diferentes orgäos de Petiveria alliacea. 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Laboratorio provincial de producción de medicamentos, Sancti Spiritus, Cuba. 39 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, 2001 Genotoxicidad in vivo: ensayo de morfología de la cabeza del espermatozoide en ratones de decocción liofilizada de hojas frescas de Petiveria alliacea L.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende, Ciudad de La Habana, Cuba. 40 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V. 2001 Genotoxicidad in vivo: ensayo de micronúcleos en médula ósea de decocción liofilizada de hoja fresca de Petiveria alliacea L. Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende, Ciudad de La Habana, Cuba. 41 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, 2001 Genotoxicidad in vitro: mediante el sistema de ensayo con Aspergillus nidulans de decocción liofilizada de hoja fresca Petiveria alliacea L. Informe TRAMIL. 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