colds | TRAMIL

	Cissus verticillata (In territories with significant traditional TRAMIL use) Dominican Republic : bejuco caro Haiti : lyann mòl Significant uses found by the TRAMIL surveys leaf, decoction with sugar, orally¹	Recommandations Preparation and posology References	According to published and other information: The topical use for abscess, ganglionar inflammation and boils is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, and skin toxicity studies. For topical application, strict hygiene measures should be observed in order to avoid contamination or additional infection. Should there be a notable worsening of the patient’s condition, or should the abscess or boils last more than 5 days, seek medical attention. Oral administration for cold, cough and influenza is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies. Not for use by women during pregnancy, during lactation or by children under 5 years old.		For abscess, ganglionar inflammation and boils: Wash injury with boiled water and soap. Apply the washed and crushed leaf in sufficient quantity to the affected area. Cover the injury with a dressing or clean cloth. Replace every 12 hours. For colds, cough and influenza: There is no available information establishing a means of preparation and dosage other than that referred to by traditional use.	1 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 2 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 3 WENIGER B, SAVARY H, DAGUIHL R, 1984 Tri phytochimique de plantes de la liste TRAMIL. Laboratoire de chimie des substances naturelles, Faculté de Médecine et de Pharmacie, Université d'Etat d'Haïti, Port au Prince, Haïti. 4 PINEDA M, 1990 Fitoquímica de algunas plantas TRAMIL. Laboratorio enda-caribe, Santo Domingo, Rep. Dominicana. 5 STANDLEY PC, 1937 The flora of Costa Rica, Pt. I. Bot Serv Field Mus Nat Hist 18(391):398. 6 TOLEDO MCF, REYES FGR, IADEROZA M, FRANCIS FJ, DRAETTA IS, 1983 Anthocyanins from anil trepador (Cissus sicyoides Linn.) J Food Sci 48(4):1368-1369. 7 MARTINEZ MJ, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2002 Evaluación de la actividad antiestafilocóccica de Cissus verticillata. Informe TRAMIL.Laboratorio Central de Farmacología, Facultad de Ciencias Médicas "Dr. Salvador Allende", La Habana, Cuba. 8 LE GRAND A, WONDERGEM PA, 1986 Activités antimicrobiennes et études bibliographiques de la toxicologie de dix plantes médicinales de la Caraïbe. Rapport TRAMIL. Dép. de Pharmacognosie, Universités de Groningen & Leyden, Hollande. 9 CAMBAR P, 1992 Efecto de los extractos acuosos de Cissus verticillata L. Informe TRAMIL. Unidad de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de Honduras UNAH, Tegucigalpa, Honduras. 10 GUARDARRAMA I, FRANCO A, ROMERO M, 1992 Estudio preliminar de las propiedades antiserotonínicas del Cissus sicyoides (Bejuco ubí). Informe TRAMIL. Instituto Superior de Ciencias Médicas, Santa Clara, Cuba. TRAMIL VI, Basse Terre, Guadeloupe, UAG/enda-caribe. 11 FENG PC, HAYNES LJ, MAGNUS KE, PLIMMER JR, 1964 Pharmacological screening of some West Indian medicinal plants. J Pharm Pharmacol 16:115-117. 12 JIU J, 1966 A survey of some medicinal plants of Mexico for selected biological activities. Lloydia 29:250-259. 13 GARCIA MD, QUILEZ AM, SAENZ MT, MARTINEZ DOMINGUEZ ME, DE LA PUERTA R, 2000. Anti-inflammatory activity of Agave intermixta Trel. and Cissus sicyoides L., species used in South Caribbean traditional medicine. J Ethnopharmacol 71(3): 395-400. 14 MARTINEZ MJ, LOPEZ M, BOUCOURT E, BETANCOURT J, FUENTES V, MORON F, 2002. Irritabilidad dérmica primaria de Cissus verticillata. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas "Dr. Salvador Allende", La Habana, Cuba. 15 MARTINEZ MJ, LOPEZ M, BOUCOURT E, BETANCOURT J, FUENTES V, MORON F, 2002. Toxicidad aguda tópica de Cissus verticillata. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas "Dr. Salvador Allende", La Habana, Cuba.
	Citrus aurantiifolia (In territories with significant traditional TRAMIL use) Colombia : limón agrio Colombia : lima Colombia : limón Dominica : lime Dominican Republic : limón, limón agrio, lima Marie-Galante Island : sitron-vè Marie-Galante Island : sitwon péyi Guadeloupe : ti sitwon vè Guadeloupe : sitwon péyi French Guiana : citron vert French Guiana : citron Honduras : limón Honduras : limón agrio Honduras : lima Haiti : ti sitwon vè Haiti : sitwon péyi St Martin : sitron-vè St Martin : sitwon péyi Martinique : sitwon péyi Martinique : sitron-vè Panama : limón agrio Panama : limón Panama : lima Puerto Rico : limón agrio Puerto Rico : lima Puerto Rico : limón Tobago : lime Venezuela : limón Venezuela : limón agrio Venezuela : lima Significant uses found by the TRAMIL surveys leaf, decoction or infusion, orally^1-3,10	Recommandations Preparation and posology References	According to published and other information: Use for conjunctivitis, headache, ear pain, fever, flu, cough and diarrhea is classified as REC, based on the significant traditional use (OMS/WHO)⁴ documented in the TRAMIL surveys. For topical application, strict hygiene measures should be observed in order to avoid contamination or additional infection, and contact with any substance that may be irritating for the conjunctiva should be avoided. In case of conjunctivitis, there is a risk of increasing irritation with the application of the Citrus spp juice. Should there be a notable worsening of the patient’s condition, or should the conjunctivitis last more than 3 days, seek medical attention. In cases of ear pain, this may be due to otitis media or interna; therefore the evaluation of a physician is recommended as the first step. Use is contraindicated in the presence of secretions in the ear and/or possible perforation of tympanum. Should there be a notable worsening of the patient’s condition, or should ear pain or fever last more than 2 days, seek medical attention. In cases of diarrhea, should there be a notable worsening of the patient’s condition, or should diarrhea last more than 3 days in adult or 2 days in children, seek medical attention. In diarrhea, the use of this resource is complementary to oral re-hydration therapy. Not for use by women during pregnancy, during lactation or by children under 5 years old. The essential oil of the plant can cause reactions of hypersensitivity reactions.		The fruit and the juice of Citrus aurantiifolia are widely used for human consumption and the peel is an industrial source of essential oil. For all reported uses: There is no available information establishing a means of preparation and dosage other than that referred to by traditional use.	1 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 2 WENIGER B, 1987-88 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 3 LAGOS-WITTE S, 1988-89, 1996 Encuesta TRAMIL. Laboratorio de Histología Vegetal y Etnobotánica, Departamento de Biología, Universidad Nacional Autónoma de Honduras UNAH, Tegucigalpa, Honduras. 4 HERRERA J,1994 Encuesta TRAMIL (Costa atlántica). Laboratorio de Fitofarmacología, Departamento de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia. 5 HAY YO,1998 Enquête TRAMIL (St. Georges). Institut de Recherche pour le Développement IRD, Cayenne, Guyane. 6 EDOUARD JA, 1992 Enquête TRAMIL. Lycée agricole, Baie-Mahault, Guadeloupe. 7 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 8 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 9 BENEDETTI MD,1994 Encuesta TRAMIL. Universidad de Puerto Rico, Mayagüez, Puerto Rico. 10 SolIs PN, Espinosa A, De Gracia J, Martínez L, Gupta MP, 2003 Encuesta TRAMIL (Emberá-Wounaann). Centro de Investigaciones Farmacognósticas de la Flora Panameña, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 11 WHO, 1991 Pautas para la evaluación de medicamentos herbarios WHO/TRM/91.4 (original inglés). Programa de Medicina Tradicional, OMS, Ginebra, Suiza. 12 EKUNDAYO O, BAKARE O, ADESOMOJU A, STAHL-BISKUP E, 1991 Volatile constituents of the leaf oil of Nigerian lime (Citrus aurantiifolia). J Essent Oil Res 3(2):119-120. 13 BEZANGER-BEAUQUESNE L, PINKAS M, TORCK M, 1986 Les plantes dans la thérapeutique moderne. 2 éd. Paris, France: Ed. Maloine. 14 DUKE JA, ATCHLEY AA, 1986 Handbook of proximate analysis tables of higher plants. Boca Raton, USA: CRC Press. p45. 15 SAUVAIN M, KODJOED JF, BERGRAVE SJ, BONNEVIE O, DEDET JP, 1986 Plantes fébrifuges en médecine traditionnelle en Haïti et en République Dominicaine et thérapie du paludisme. Rapport TRAMIL. ORSTOM, Cayenne, Guyane Française. 16 NOGATA Y, YOZA KI, KUSUMOTO KI, KOHYAMA N, SEKIYA K, OHTA H, 1996 Screening for inhibitory activity of Citrus fruit extracts against platelet cyclooxygenase and lipoxygenase. J Agric Food Chem 44(3):725-729. 17 CACERES A, GIRON L, ALVARADO S, TORRES MF, 1987 Screening of antimicrobial activity of plants popularly used in Guatemala for the treatment of dermatomucosal diseases. J Ethnopharmacol 20(3):223-237. 18 EBANA RU, MADUNAGU BE, EKPE ED, OTUNG IN, 1991 Microbiological exploitation of cardiac glycosides and alkaloids from Garcinia kola, Borreria ocymoides, Kola nitida and Citrus aurantiifolia. J Appl Bacteriol 71(5):398-401. 19 KOICHUSAKUL S, SATHITNIRAIMAI S, 1977 Studies of the effect of sour fruits on acid secretion in the stomach. Undergraduate special problem report. Fac Med (Siriraj Hosp) Mahidol Univ, Bangkok, Thailand. 20 DHAWAN BN, PATNAIK GK, RASTOGI RP, SINGH KK, TANDON JS, 1977 Screening of Indian plants for biological activity. VI. Indian J Exp Biol 15(3):208-219. 21 LAM L, ZHENG B, 1991 Effects of essential oils on glutathione S-transferase activity in mice. J Agric Food Chem 39(4):660-662. 22 EL KELTAWI N, MEGALLA S, ROSS S, 1980 Antimicrobial activity of some Egyptian aromatic plants. Herbal Pol 26(4):245-250. 23 ADESINA S, 1982 Studies on some plants used as anticonvulsants in Amerindian and African traditional medecine. Fitoterapia 53:147-162. 24 GUPTA M, 1987 Essential oil: a new source of bee repellents. Chem Ind (London) 5:161-163. 25 HARTMAN JG, LIMBIRD ILE, Eds., 1996 Goodman & Gilman Las bases farmacológicas de la terapéutica, 9^a ed. México, México:Mc Graw-Hill Interamericana. 26 BALA S, GROVER IS, 1989 Antimutagenicity of some Citrus fruits in Salmonella typhimurium. Mutat Res 222(3):141-148. 27 PELLECUER J, 1995 Aromaterapia y toxicidad de los aceites esenciales. Natura Medicatrix 37(8):36-40. 28 Olmedo D, RODRIGUEZ N, ESPINOSA A, VASQUEZ Y, Gupta MP, 2005 Ensayo antimicrobiano de algunas especies con usos significativos TRAMIL-Centroamérica. Informe TRAMIL. Centro de Investigaciones Farmacognósticas de la Flora Panameña CIFLORPAN, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 29 MARTINEZ MJ, MOREJON Z, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2005 Clases tóxicas agudas (CTA) de una decocción de corteza de fruto fresco de Citrus aurantiifolia (Christm.) Swing. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 30 MARTINEZ MJ, MOREJON Z, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2005. Clases tóxicas agudas (CTA) de una decocción de hoja fresca de Citrus aurantiifolia (Christm.) Swing. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 31 GarcIa-GONZÁLEZ M, BARBOZA CJ. 2005 Toxicidad aguda (5000 mg/kg) dosis repetida, en ratones, del extracto acuoso de hojas frescas de Citrus aurantiifolia. Informe TRAMIL GEF/UNEP.PRONAPLAMED. Depto de Fisiología, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 32 GarcIa-GONZÁLEZ M, BARBOZA CJ. 2005 Velocidad del tránsito intestinal en ratones, del extracto acuoso del fruto fresco de Citrus aurantiifolia. Informe TRAMIL. PRONAPLAMED. Depto de Fisiología, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 33 GarcIa-GONZÁLEZ M, BARBOZA CJ. 2005 Velocidad del tránsito intestinal en ratones, del extracto acuoso de hojas frescas de Citrus aurantiifolia. Informe TRAMIL. PRONAPLAMED. Depto de Fisiología, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 34 DELAIGUE J, 2005 TRAMIL survey. UAG & PRDI, Tobago House of Assembly, Scarborough, Tobago. 35 ZambranoLE, 2007 Encuesta TRAMIL en Guareguare, Miranda. UCV, Caracas, Venezuela. 36 OCRISSE G, 2008 Enquête TRAMIL auprès de 250 familles de la moitié Est de la partie francophone de St Martin. Biologie végétale, UAG, Guadeloupe. 37 BALZ E, BOYER A, BURAUD M, 2007 Enquête TRAMIL à Marie-Galante. U. Bordeaux 3, U. Paris XI Chatenay-Malabry, UAG, Guadeloupe. 38 MARTINEZ MJ, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, MORON F, 2007 Irritabilidad dérmica (piel sana) primaria de zumo fresco de fruto de Citrus aurantiifolia (Christm) Swing var mexicana.Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 39 PAZOS L, COTO T, CAIZA F, 2009 Irritación ocular, en conejos, del jugo fresco del fruto de Citrus aurantiifolia. Informe TRAMIL. Laboratorio de Ensayos Biológicos, LEBi, Universidad de Costa Rica, San Pedro, Costa Rica. 40 PAZOS L, COTO T, CAIZA F, 2009 Toxicidad oral aguda, dosis repetida, en ratón, hoja fresca de Citrus aurantiifolia. Informe TRAMIL. Laboratorio de Ensayos Biológicos, LEBi, Universidad de Costa Rica, San Pedro, Costa Rica. 41 PAZOS L, COTO T, CAIZA F, 2009 Toxicidad oral aguda, dosis repetida, en ratón, cáscara del fruto fresco de Citrus aurantiifolia. Informe TRAMIL. Laboratorio de Ensayos Biológicos, LEBi, Universidad de Costa Rica, San Pedro, Costa Rica. 42 LONGUEFOSSE JL, NOSSIN E, 1990-95 Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique. 43 FRIAS AI, GARCIA N, MOREJON Z, MORON F, VICTORIA MC, 2009 Efecto antiinflamatorio tópico del zumo puro del fruto fresco de Citrus aurantiifolia (Christm.) Swingle (limón) en el edema de la oreja inducido por aceite de Croton en ratones. Trabajo TRAMIL. Laboratorio Central de Farmacología. Universidad de Ciencias Médicas de La Habana. 45 BOULOGNE I, 2009 Enquête TRAMIL, (Terre-de-Bas et Terre-de-Haut) Les Saintes, UAG, Guadeloupe. 46 LOPEZ M, MOREJON Z, MARTINEZ MJ, BACALLAO Y, FUENTES V, MORON F, 2009 Irritabilidad dérmica piel lesionada, dosis repetida de Citrus aurantifolia (Christm) Swing. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, Cuba, C. Habana.
	Cymbopogon citratus (In territories with significant traditional TRAMIL use) Antigua : fever grass Antigua : lemon grass Barbados : fever grass Barbados : lemon grass Costa Rica : zacate limón Costa Rica : té limón Costa Rica : zacate té Dominica : sitwonnèl Dominica : zacate té Dominican Republic : limoncillo Marie-Galante Island : sitwonnèl Marie-Galante Island : zacate té Guatemala : té de limón Honduras : zacate limón Honduras : té limón Honduras : zacate té Saint Lucia : sitwonnèl Saint Lucia : zacate té St Martin : sitwonnèl St Martin : zacate té Martinique : sitwonnèl Martinique : zacate té Quintana Roo : té limón Quintana Roo : zacate té Quintana Roo : zacate limón Puerto Rico : limoncillo Tobago : lemon grass Tobago : fever grass Saint Vincent : fever grass Saint Vincent : lemon grass Venezuela : molojillo criollo Significant uses found by the TRAMIL surveys leaf, decoction or infusion, orally^{4-5,8,11-12,44-46,48}	Recommandations Preparation and posology References	According to published and other information: Use for diarrhea, stomach pain, fever, flatulence, flu, colds and cough is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies, scientific validation and available published scientific information. In case of diarrhea, the use of this resource can be considered complementary to oral re-hydration therapy. Should there be a notable worsening of the patient’s condition, or should diarrhea last more than 3 days in adult or 2 days in children, or should fever last more than 2 days, stomach pain more than 3, or cough more than 5, seek medical attention. Not for use during pregnancy, during lactation or by children under 3 years old.		TRAMIL Research⁴² For diarrhea, stomach pain, fever, flatulence, flu, colds and cough: Prepare a decoction or infusion with 15-25 grams of leaf in 1 liter (4 cups) of water. For decoction, boil for at least10 minutes in a covered pot; for infusion, add boiling water to 15-25 grams of leaf. Cover and leave to cool down. Filter and drink 1 cup (250 mL), 2-3 times a day. In all the above-mentioned uses for oral administration, the preparation should be properly filtered, using a cloth, as a prerequisite for consumption, in order to avoid mechanical injuries to the mucosas, due to the microfilaments present in the leaf²⁶.	1 LAGOS-WITTE S, 1988-89, 1996 Encuesta TRAMIL. Laboratorio de Histología Vegetal y Etnobotánica, Dep. de Biología, Universidad Nacional Autónoma de Honduras UNAH, Tegucigalpa, Honduras. 2 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 3 GIRON L, 1988 Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala. 4 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 5 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 6 JEAN-PIERRE L, 1988 TRAMIL survey. St. Lucia National Herbarium, Castries, St. Lucia. 7 FAUJOUR A, MURREY D, CHELTENHAM-CORBIN B, CARRINGTON S, 2003 TRAMIL survey. enda-caribbean, IICA & UAG, Saint Thomas, Barbados. 8 OCAMPO R, 1988 Encuesta TRAMIL (Costa atlántica), Instituto de Desarrollo Agrario, Universidad de Costa Rica, San José, Costa Rica. 9 LONGUEFOSSE JL, NOSSIN E, 1990-95 Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique. 10 DELENS M, 1992 Encuesta TRAMIL en los Estados Lara y Sucre de Venezuela. Centro al Servicio de la Acción Popular CESAP, Caracas, Venezuela. 11 O'REILLY A, 1992 TRAMIL survey. Chemistry & Food Technology Division, Ministry of Agriculture, Dunbars, Antigua & Barbuda. 12 BENEDETTI MD, 1994 Encuesta TRAMIL. Universidad de Puerto Rico, Mayagüez, Puerto Rico. 13 MENDEZ M, MEDINA ML, DURAN R, 1996 Encuesta TRAMIL. Unidad de recursos naturales, Centro de Investigación Científica de Yucatán CICY, Mérida, México. 14 BALLAND V, GLASGOW A, SPRINGER F, GAYMES G, 2004 TRAMIL survey. IICA, UAG & U.PARIS XI, Saint Vincent. 15 ABEGAZ B, YOHANNES P, DIETER R, 1983 Constituents of the essential oil of Ethiopian Cymbopogon citratus. J Nat Prod 46(3):424-426. 16 DE MATOUSCHEK B, STAHL-BISKUP E, 1991 Phytochemical investigation of nonvolatile constituents ofCymbopogon citratus (DC.) Stapf. (Poaceae). Pharm Acta Helv 66(9/10):242-245. 17 HANSON S, CRAWFORD M, KOKER M, MENEZES F, 1976 Cymbopogonol, a new triterpenoid from Cymbopogon citratus. Phytochemistry15:1074-1075. 18 YOKOYAMA Y, TSUYUKI T, NAKAMURA N, TAKAHASHI T, HANSON S, MATSUSHITA K, 1980 Revised structures of cymbopogone and cymbopogonol. Tetrahedron Lett21:3701-3702. 19 OLANIYI A, SOFOWORA E, OGUNTIMEHIN B, 1975 Phytochemical investigation of some Nigerian plants used against fevers. II. Cymbopogon citratus. Planta Med 28:186-189. 20 WILLAMAN JJ, LI H, 1970 Alkaloid-bearing plants and their contained alkaloids, 1957-1968. Lloydia33(Supp.3A):1-286. 21 DUKE JA, ATCHLEY AA, 1986 Handbook of proximate analysis tables of higher plants. Boca Raton, USA: CRC Press. p57. 22 SAUVAIN M, MORETTI C, MUÑOZ V, 1990 Pruebas in vivo para paludismo realizadas en Bolivia sobre varias plantas TRAMIL. ORSTOM/IRD/IBBA, La Paz, Bolivia. 23 MORON F, SANCHEZ C, MARTINEZ MC, MOREJON Z, PINEDO Z, 2000 Actividad antiespasmódica in vitro de hojas frescas de Cymbopogon citratus (DC.) Stapf. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 24 MORON F, FURONES J, PINEDO Z, 1996 Ausencia de efectos antiinflamatorio y analgésico del extracto fluído de Cymbopogon citratus al 30% por vía oral. Rev Cubana Plant Med 1(2):3-6. 25 CARBALLO A, 1995 Plantas medicinales del Escambray cubano. Informe TRAMIL. Laboratorio provincial de producción de medicamentos, Sancti Spiritus, Cuba. 26 WENIGER B, ROUZIER M, DAGUILH R, HENRYS D, HENRYS J, ANTON R, 1986 Popular medicine of the central plateau of Haiti. 2. Ethnopharmacological inventory. J Ethnopharmacol 17(1):13-30. 27 CARLINI EA, CONTAR JD, SILVA-FILHO AR, SOLVEIRA-FILHO NG, FROCHTENGARTEN ML, BUENO OF, 1986 Pharmacology of lemon-grass Cymbopogon citratus I. Effect of teas prepared from the leaves on laboratory animals. J Ethnopharmacol 17(1):37-64. 28 SOUZA FORMIGONI ML, LODDER HM, FILHO OG, FERREIRA TM, CARLINI EA, 1986 Pharmacology of lemongrass (Cymbopogon citratus Stapf). II. Effects of daily two month administration in male and female rats and in offspring exposed "in utero". J Ethnopharmacol 17(1):65-74. 29 CARBAJAL D, CASACO A, ARRUZAZABALA L, GONZALEZ R, TOLON Z, 1989 Pharmacological study of Cymbopogon citratus leaves. J Ethnopharmacol25(1):103-107. 30 LAM L, ZHENG B, 1991 Effects of essential oils on glutathione S-transferase activity in mice. J Agric Food Chem 39(4):660-662. 31 LORENZETTI B, SOUZA G, SARTI S, FILHO DS, FERREIRA SH, 1991 Myrcene mimics the peripheral analgesic activity of lemongrass tea. J Ethnopharmacol 34(1):43-48. 32 LEMOS TLG, MATOS FJA, ALENCAR JW, CRAVEIRO AA, CLARK AM, MC CHESNEY JD, 1990 Antimicrobial activity of essential oils of Brazilian plants. Phytother Res4(2):82-84. 33 AWUAH R, 1989 Fungitoxic effects of extracts from some West African plants. Ann Appl Biol 115(3):451-453. 34 REYNOLDS JEF, PRASAD AB, Eds., 1982 MARTINDALE The extra pharmacopoeia. 28th ed. London, England: The Pharmaceutical Press. p677. 35 SETH, G, KOKATE CK, VARMA KC, 1976 Effect of essential oil of Cymbopogon citratus on central nervous system. Indian J Exp Biol 14(3):370-371. 36 DUKE JA, 1992 Handbook of biologically active phytochemicals and their bioactivities. Boca Raton, USA: CRC Press. 37 KOBAYASHI N, 1989 Pharmaceutical compositions containing lemongrass extracts and antioxidants. Patens Japan Kokai Tokio Koho., 01, 221, 320. 38 MARTINEZ MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BARCELO H, LAINEZ A, MONTES ME, REGO R, BOUCOURT E, MORON F, 2000 Toxicidad aguda clásica de hoja seca de Cymbopogon citratus (DC.) Stapf.Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 39 MARTINEZ MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, MORON F, 2000 Actividad genotóxica in vitro de hoja seca de Cymbopogon citratus (DC.) Stapf. Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 40 de la Torre RA, Espinosa-Aguirre JJ, Cortinas de Nava C, Izquierdo T, Moron F, 1994 Genotoxic activity of mebendazole in Aspergillus nidulans. Mutat Res 305(2):139-144. 41 LEITE JR, SEABRA ML, MALUF E, ASSOLANT K, SUCHECKI D, TUFIK S, KLEPACZ S, CALIL HM, CARLINI EA, 1986 Pharmacology of lemongrass (Cymbopogon citrates Stapf). III. Assessment of eventual toxic, hypnotic and anxiolytic effects on humans. J Ethnopharmacol 17(1):75-83. 42 CARBALLO A, 1995 Cálculo de concentración y dosis de las drogas vegetales TRAMIL: Mensuraciones farmacognósticas y aproximaciones técnico-clínicas. Laboratorio provincial de producción de medicamentos, Sancti Spiritus, Cuba. 43 DELAIGUE J, 2005 TRAMIL survey. UAG & PRDI, Tobago House of Assembly, Scarborough, Tobago. 44 ZambranoLE, 2007 Encuesta TRAMIL en Guareguare, Miranda. UCV, Caracas, Venezuela. 45 BALZ E, BOYER A, BURAUD M, 2007 Enquête TRAMIL à Marie-Galante. U. Bordeaux 3, U. Paris XI Chatenay-Malabry, UAG, Guadeloupe. 46 BOYER A, BURAUD M, 2007 Enquête TRAMIL à La Désirade. U. Paris XI Chatenay-Malabry, UAG, Guadeloupe. 47 OCRISSE G, 2008 Enquête TRAMIL auprès de 250 familles de la moitié Est de la partie francophone de St Martin. Biologie végétale, UAG, Guadeloupe. 48 BOULOGNE I, 2009 Enquête TRAMIL, (Terre-de-Bas et Terre-de-Haut) Les Saintes, UAG, Guadeloupe.
	Lippia alba (In territories with significant traditional TRAMIL use) Saint Martin : ti bouch Martinique : lamerik Martinique : brisée Panama : mastranto Puerto Rico : poleo Venezuela : Santa María Venezuela : toronjil Venezuela : malojillo extranjero Venezuela : poleo Significant uses found by the TRAMIL surveys fresh leaf, decoction, orally³	Recommandations Preparation and posology References	According to published and other information: Uses for flu and common cold are classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies and available published scientific information. Should there be a notable worsening of the patient’s condition, or should flu or common cold symptoms persist for more than 7 days, seek medical attention. Not for use during pregnancy, during lactation or by children under 5 years old.		For flu and common cold: Prepare an infusion: add 1 cup (250 mL) of boiling water to 7.5 grams of previously washed and chopped fresh leaves (approximately 20 medium-sized leaves) or aerial parts. Cover the pot and let the preparation settle for 5-10 minutes. Filter and drink 1 cup 3 times a day.	1 LONGUEFOSSE JL, NOSSIN E, 1990-95 Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique. 2 DELENS M, 1992 Encuesta TRAMIL en los Estados Lara y Sucre de Venezuela. Centro al Servicio de la Acción Popular CESAP, Caracas, Venezuela. 3 SOLIS P, CORREA M, GUPTA M, 1995 Encuesta TRAMIL (Comunidades afro-caribeñas). Centro de Investigaciones Farmacognósticas de la Flora Panameña CIFLORPAN, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 4 CATALAN C, MEREP D, RETAMAR J, 1977 The essential oil of Lippia alba from the Tucuman province. Riv Ital Essenze Profumi Piante Offic Aromi Saponi Cosmet Aer 59: 513-518. 5 FESTER G, MARTINUZZI E, RETAMAR J, RICCIARDI A, 1955 Some volatile essential oils. VII. Rev Fac Ing Quim 24: 37-55. 6 CASTRO DM, MING LC, MARQUES MO, TANAKA FA, 2000 Anatomic characterization and chemical composition of essential oils of different leaves of Lippia alba (Mill.) stem axis. 1er Congreso Peruano de Plantas Medicinales y Fitoterapía, 27-30 Septiembre 2000, Lima, Perú:112-114. 7 BANDONI A, 2003 Los recursos vegetales aromáticos en Latinoamérica: su aprovechamiento industrial para la producción de aromas y sabores. Buenos Aires, Argentina: CYTED, 2 ed., CD-ROM, ISBN:987-43-6072-0. 8 FISCHER U, LOPEZ R, POLL E, VETTER S, NOVAK J, FRANZ CM, 2004 Two chemotypes within Lippia alba populations in Guatemala. Flavour and Fragrance Journal 19(4):333-335. 9 BANDONI A, MENDIONDO M, RONDINA R, COUSSIO J, 1976 Survey of Argentine medicinal plants. Folklore and phytochemical screening. II. Econ Bot 30: 161-185. 10 CACERES A, ALVAREZ AV, OVANDO AE, SAMAYOA BE, 1991 Plants used in Guatemala for the treatment of respiratory diseases. 1. Screening of 68 plants against gram-positive bacteria. J Ethnopharmacol 31(2):193-208. 11 RUFFA MJ, WAGNER ML, SURIANO M, VICENTE C, NADINIC J, PAMPURO S, SALOMON H, CAMPOS RH, CAVALLARO L, 2004 Inhibitory effect of medicinal herbs against RNA and DNA viruses. Antivir Chem Chemother 15(3):153-159. 12 ABAD MJ, BERMEJO P, VILLAR A, PALOMINO SS, CARRASCO L, 1997 Antiviral activity of medicinal plant extracts. Phytother Res 11(3):198-202. 13 PASCUAL ME, SLOWING K, CARRETERO ME, VILLAR A, 2001 Antiulcerogenic activity of Lippia alba (Mill.) N. E. Brown (Verbenaceae). Farmaco. 56(5-7):501-504. 14 COSTA M, DI STASI L, KIRIZAWA M, MENDACOLLI S, GOMES C, TROLIN G, 1989 Screening in mice of some medicinal plants used for analgesic purposes in the state of Sao Paulo. J Ethnopharmacol 27(1/2):25-33. 15 VALE TG, MATOS FJA, DE LIMA TCM, VIANA GSB, 1999 Behavioral effects of essential oils from Lippia alba (Mill.) N.E. Brown chemotypes. J Ethnopharmacol 167:127-133. 16 CARBALLO A, 1994 Plantas medicinales del Escambray Cubano. Apuntes científicos. 17 GarcIa GM, Coto MT, GonzAlez CS, OCAMPO R, Pazos L, 2001 Toxicidad aguda en ratones, del extracto acuoso de hoja fresca de Lippia alba. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBI, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 18 PAZOS L, COTO T, GONZALEZ S, 2003 Toxicidad oral, aguda en ratón, del extracto acuoso de partes aéreas de Lippia alba var. dulce. Informe TRAMIL. Laboratorio de Ensayos Biológicos, Universidad de Costa Rica, San Pedro, Costa Rica. 19 PAZOS L, COTO T, GONZALEZ S, QUIROS S, 2004 Toxicidad oral, aguda en ratón, dosis repetidas, del extracto acuoso de partes aéreas de Lippia alba. Informe TRAMIL.Laboratorio de Ensayos Biológicos, Universidad de Costa Rica, San Pedro, Costa Rica. 20 BHAKUNI D, DHAR M, DHAR M, DHAWAN B, GUPTA B, SRIMALI R, 1971 Screening of Indian plants for biological activity. Part III. Indian J Exp Biol 9:91. 21 Code of Federal Regulations, 2004 Food and drugs. Chapter I - Food and Drug Administration, Department of Health and Human services. Part 182 - Substances generally recognized as safe. Sec. 182.10. Spices and other natural seasonings and flavorings. U.S. Government Printing Office via GPO Access, USA. 21(3):451-452. Nov.13,2004, URL: www.cfsan.fda.gov/~lrd/FCF182.html 22 BENEDETTI MD, MEJIA A, ACOSTA D, 1994 Encuesta TRAMIL. Universidad de Puerto Rico, Mayagüez, Puerto Rico.
	Mentha sp. (In territories with significant traditional TRAMIL use) St Martin : mant Dominica : peppermint Marie-Galante Island : mant Cuba : toronjil de menta Cuba : toronjil Guadeloupe : mant Martinique : mant Panama : yerba buena Venezuela : yerba buena Significant uses found by the TRAMIL surveys leaf, decoction or infusion, orally^5-6,38	Recommandations Preparation and posology References	According to published and other information: Use for diarrhea, stomach pain, flatulence, indigestion, flu, common cold and vomiting is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, and on available published scientific information. Should there be a notable worsening of the patient’s condition, or should the diarrhea or stomach pain last more than 3 days in adults or 2 days in children older than 3, or should vomiting persist for more than 2 days, seek medical attention. For diarrhea, the use of this resource can be considered complementary to oral re-hydration therapy. Do not ingest in case of adverse gall bladder conditions or stones⁷. Not for use during pregnancy, during lactation or by children under 5 years old.		The leaf of Mentha spicata is widely used for human consumption andMentha piperita is an industrial source of essential oil. For stomach ache: Prepare an infusion adding 250 mL (1 cup) of boiling water to 1.5-3 grams (1 spoonful of dried leaf. Cover pot, let it settle for 5-10 minutes, and filter. For diarrhea, flatulence, indigestion, flu, common cold and vomiting: Prepare a decoction or infusion with 1.5-3 grams (1 spoonful) of dried leaf in 250 mL (1 cup) of water. In the case of a decoction, boil for at least 10 minutes in a covered pot; for infusion, add boiling water to 3 grams of dried leaf, cover, leave to cool down for 5-10 minutes, and filter. In all cases, drink 2-4 cups a day when required by symptomatic indication^32-33.	1 DELENS M, 1990-92 Encuesta TRAMIL. Centro al Servicio de la Acción Popular CESAP, Caracas, Venezuela. 2 CARBALLO A, 1990 Encuesta TRAMIL. Centro de investigación de fitoterapia y medicina tradicional de Topes de Collantes, Trinidad, Cuba. 3 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 4 EDOUARD JA, 1992 Enquête TRAMIL. Lycée agricole, Baie-Mahault, Guadeloupe. 5 LONGUEFOSSE JL, NOSSIN E, 1990-95 Enquête TRAMIL. Association pour la valorisation des plantes médicinales de la Caraïbe AVPMC, Fort de France, Martinique. 6 SOLIS P, CORREA M, GUPTA M, 1995 Encuesta TRAMIL (Comunidades afro-caribeñas). Centro de Investigaciones Farmacognósticas de la Flora Panameña CIFLORPAN, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 7 CANIGUERAL S, VILA R, RISCO E, PEREZ F, PORTILLO A, FREIXA B, MILO B, VANACLOCHA B, RIOS JL, MORALES MA, ALONSO JR, BACHILLER LI, PERIS JB, STUBING G, 2002 Mentha sp. Vademecum de Fitoterapia, Editorial Masson, Barcelona, España, Nov. 20, 2003. URL: http://www.masson.es/book/fitoterapia.html 8 TAYLOR BA, DUTHIE HL, LUSCOMBE DK, 1985 Mechanism by which peppermint oil exerts its relaxant effect on gastrointestinal smooth muscle. J Pharm Pharmacol 37(Suppl):104. 9 GUEDON DJ, PASQUIER BP, 1994 Analysis and distribution of flavonoid glycosides and rosmarinic acid in 40 Mentha xpiperita clones. J Agr Food Chem 42(3):679-684. 10 HERRMANN EC, KUCERA LS, 1967 Antiviral substances in plants of the mint family (Labiatae). 3. Peppermint (Mentha piperita) and other mint plants. Proc Soc Exp Biol Med 124(3):874-878. 11 KIUCHI F, NAKAMURA N, MIYASHITA N, NISHIZAWA S, TSUDA Y, KONDO K, 1989 Nematocidal activity of some anthelmintic traditional medicines and spices by a new assay method using larvae of Toxocara canis. Shoyakugaku Zasshi 43(4):279-287. 12 LESLIE GB, 1978 A pharmacometric evaluation of nine bio-strath herbal remedies. Medita 8(10):3-19. 13 DELLA LOGGIA R, TUBARO A, LUNDER TL, 1990 Evaluation of some pharmacological activities of a peppermint extract. Fitoterapia 61(3):215-221. 14 DELLA LOGGIA R, TUBARO A, REDAELLI C, 1981 Valutazione dell'attività sul S.N.C. del topo di alcuni estratti vegetali e di una loro associazione. (Evaluation of the activity on the mouse CNS of several plant extracts and a combination of them). Rivista di Neurologia 51(5):297-310. 15 COSTA M, DI STASI LC, KIRIZAWA M, MENDACOLLI SL, GOMES C, TROLIN G, 1989 Screening in mice of some medicinal plants used for analgesic purposes in the state of Sao Paulo. Part II. J Ethnopharmacol 27(1-2):25-33. 16 ROSS S, EL-KELTAWI N, MEGALLA S, 1980 Antimicrobial activity of some Egyptian aromatic plants. Fitoterapia 51:201-205. 17 SCORTICHINI M, ROSSI M, 1989 In vitro activity of some essential oils toward Erwinia amylovora (Burril) Winslow. Acta Phytopathol Entomol Hung 24(3/4):421-431. 18 RAI MK, UPADHYAY S, 1988 Laboratory evaluation of essential oil of Mentha piperita Linn. against Trichophyton mentagrophytes. Hindustan Antibiot Bull 30(3-4):82-84. 19 HARRIES N, JAMES KC, PUGH WK, 1978 Antifoaming and carminative actions of volatile oils. J Clin Pharmacol 2:171-177. 20 TADDEI I, GIACHETTI D, TADDEI E, MANTOVANI P, BIANCHI E, 1988 Spasmolytic activity of peppermint, sage and rosemary essences and their major constituents. Fitoterapia 59(6):463-468. 21 MELZIG M, TEUSCHER E, 1991 Investigations of the influence of essential oils and their main components on the adenosine uptake by cultivated endothelial cells. Planta Med 57(1):41-42. 22 LAM L, ZHENG B, 1991 Effects of essential oils on glutathione S-transferase activity in mice. J Agr Food Chem 39(4):660-662. 23 BRIGGS C, 1993 Peppermint: medicinal herb and flavouring agent. Can Pharmaceutical J 126(2):89-92. 24 DALVI SS, NADKARNI PM, PARDESI R, GUPTA KC, 1991 Effect of peppermint oil on gastric emptying in man: A preliminary study using a radiolabelled solid test meal. Indian J Physiol Pharmacol 35(3):212-214. 25 MAY B, KUNTZ HD, KIESER M, KOHLER S, 1996 Efficacy of a fixed peppermint oil/caraway oil combination in non-ulcer dyspepsia. Arzneimittel Forschung [Drug Research] 46(12):1149-1153. 26 BEZIAT M, 1983 Toxicité d'huiles essentielles. Thèse Pharmacie, Montpellier, France. 27 CODE OF FEDERAL REGULATIONS, 2002 Food and drugs. Chapter I - Food and Drug administration, Department of Health and Human Services. Part 182 - Substances generally recognized as safe. Sec. 182.10. Spices and other natural seasonings and flavorings. U.S. Government Printing Office via GPO Access, USA. 21(3):451-452. Feb. 24, 2003, URL: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CF... 28 VIZOSO A, RAMOS A, VILLAESCUSA A, DECALO M, BETANCOURT J, 1997 Estudio genotóxico in vitro e in vivo en tinturas de Melissa officinalis L. (toronjil) y Mentha piperita L. (toronjil de menta). Rev Cub Plantas Med 2(1):6-11. 29 MICROMEDEX T, 2003 Healthcare Series. Vol. 117. 9/2003 Thomson MICROMEDEX^®. 30 BUDAVARI S (Ed.), 2001 The Merck index: an encyclopedia of chemical, drugs, and biologicals. 30^th ed. New Jersey, USA: Merck and Co., Inc. p1043-1044. 31 SIVASWAMY SM, BALACHANDRAN B, BALANEHRU S, SIVARAMAKRISHNAN VM, 1991 Mutagenic activity of south Indian food items. Indian J Exp Biol 29(8):730-737. 32 WICHTL M, 1999 Plantes thérapeutiques. Tec and Doc. p365. 33 ALONSO J, 1998 Tratado de fitomedicina: bases clínicas y farmacológicas. Buenos Aires, Argentina: ISIS ediciones SRL. p721. 34 MARTINEZ MJ, MOREJON Z, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2005 Clases tóxicas agudas (CTA) de una decocción de hoja fresca de Mentha nemorosa Willd.Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 35 GUERRA MJ, LOPEZ M, BOUCOURT E, FUENTES V, MORON F, 2002 Clases tóxicas agudas en rata de decocción (30%) de hojas secas de Mentha x piperita varcitrata (Ehrh.) Briq.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende. Ciudad de La Habana, Cuba. 36 GUERRA MJ, LOPEZ M, BOUCOURT E, FUENTES V, 2002 Toxicidad aguda (DL₅₀) en ratón de la decocción de hojas secas de Mentha x piperita var citrata(Ehrh.) Briq.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende. Ciudad de La Habana, Cuba. 37 BALZ E, BOYER A, BURAUD M, 2007 Enquête TRAMIL à Marie-Galante. U. Bordeaux 3, U. Paris XI Chatenay-Malabry, UAG, Guadeloupe. 38 OCRISSE G, 2008 Enquête TRAMIL auprès de 250 familles de la moitié Est de la partie francophone de St Martin. Biologie végétale, UAG, Guadeloupe.
	Momordica charantia (In territories with significant traditional TRAMIL use) Antigua : maiden blush Barbados : cerasee Costa Rica : sorosí Dominica : kokouli Dominican Republic : cundeamor Guatemala : sorosí Honduras : calaica Haiti : asorosi Puerto Rico : cundeamor Tobago : corailee Tobago : sorrow seed Tobago : popololo Venezuela : cundeamor Significant uses found by the TRAMIL surveys aerial parts, decoction, orally^10-12	Recommandations Preparation and posology References	According to published and other information: Use for furuncles and common cold is classified as REC, based on the significant traditional use documented in the TRAMIL surveys, toxicity studies and available published scientific information. Use for dry skin conditions, itching, lice (pediculosis) and burns is classified as REC, based on the significant traditional use (OMS/WHO)⁴ documented in the TRAMIL surveys. For topical application, strict hygienic measures should be observed in order to avoid contamination or additional infection. Should there be a notable worsening of the patient’s condition, or should furuncles last more than 5 days, seek medical attention. Do not take orally during pregnancy due to risk of abortion. Not for use during lactation or by children under 3 years old.		For skin conditions: Wash the aerial parts of the plant thoroughly and crush them. Apply 30 grams (a handful) of vegetal material on the affected area of skin 3 times a day. For common cold: There is no available information establishing a means of preparation and dosage other than that referred to by traditional use.	1 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 2 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 3 LAGOS-WITTE S, 1988-1995 Encuestas TRAMIL. Laboratorio de Histología Vegetal y Etnobotánica, Departamento de Biología, Universidad Nacional Autónoma de Honduras UNAH, Tegucigalpa, Honduras. 4 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 5 WENIGER B, 1987-88 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 6 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (Zambrana, Cotuí). Dep. de Botánica, Jardín BotánicoNacional, Santo Domingo, República Dominicana. 7 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (region Este). Dep. de Botánica, Jardín BotánicoNacional, Santo Domingo, República Dominicana. 8 DELENS M, 1990 Encuesta TRAMIL en Barlovento, Edo. Miranda de Venezuela. Centro al Servicio de la Acción Popular CESAP, Caracas, Venezuela. 9 GIRON L, 1988 Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala. 10 O'REILLY A, 1992 TRAMIL survey. Chemistry & Food Technology Division, Ministry of Agriculture, Dunbars, Antigua & Barbuda. 11 OCAMPO R, 1988 Encuesta TRAMIL (Costa atlántica), Instituto de Desarrollo Agrario, Universidad de Costa Rica, San José, Costa Rica. 12 FAUJOUR A, MURREY D, CHELTENHAM-CORBIN B, CARRINGTON S, 2003 TRAMIL survey. IICA & UAG, Saint Thomas, Barbados. 13 BENEDETTI MD, 1994 Encuesta TRAMIL. Universidad de Puerto Rico, Mayagüez, Puerto Rico. 14 WHO, 1991 Pautas para la evaluación de medicamentos herbarios WHO/TRM/91.4 (original inglés). Programa de Medicina Tradicional, OMS, Ginebra, Suiza. 15 WENIGER B, SAVARY H, DAGUIHL R, 1984 Tri phytochimique de plantes de la liste TRAMIL. Laboratoire de chimie des substances naturelles, Faculté de médecine et de pharmacie, Université d'Etat d'Haïti, Port au Prince, Haïti. 16 HERRERA J, 1990 Determinación de actividades biológicas de vegetales utilizados en medicina tradicional. Informe TRAMIL. Dep. de Farmacología, Facultad de Salud, Universidad del Valle, Cali, Colombia. 17 DUKE JA, ATCHLEY AA, 1986 Handbook of proximate analysis tables of higher plants. Boca Raton, USA: CRC Press. p110. 18 YASUDA M, IWAMOTO M, OKABE H, YAMAUCHI T, 1984 Structures of momordicines I, II and III, the bitter principles in the leaves and vines of Momordica charantia. Chem Pharm Bull 32(5):2044-2047. 19 DHALLA NS, GUPTA KC, SASTRY MS, MALHOTRA CL, 1961 Chemical composition of the fruit of Momordica charantia. Indian J Pharmacy 23:128-130. 20 KANNA B, 1976 Insulin from Momordica charantia. Patent-Japan Kokai. 21 NG TB, YEUNG HW, 1984 Bioactive constituents of Cucurbitaceae plants with special emphasis on Momordica charantia and Trichosanthes kirilowii. Seoul, Korea: Proc. 5^th. Symposium Medicinal Plants and Spices. 22 KHANNA P, MOHAN S, 1973 Isolation and identification of diosgenin and sterols from fruits and in vitro cultures ofMomordica charantia. Indian J Exp Biol11:58-60. 23 OKABE H, MIYAHARA K, YAMAGUCHI T, MIYAHARA K, KAWASAKI T, 1980 Studies on the constituents ofMomordica charantia L. I: Isolation and characterization of momordicosides A and B, glycosides of a pentahydroxy-cucurbitane triterpene. Chem Pharm Bull28(9):2753-2762. 24 RODRIGUEZ DB, RAYMUNDO LC, TUNG-CHING LEE, SIMPSON KL, CHICHESTER CO, 1976 Carotenoid pigment changes in ripening Momordica charantia fruits. Ann Bot (London)40:615-624. 25 LIN JY, HOU MJ, CHEN YC, 1978 Isolation of toxic and non-toxic lectins from the bitter pear melon (Momordica charantia). Toxicon16:653. 26 IYER RI, NAGAR PK, SIRCAR PK, 1981 Endogenous cytokinins in seeds of bittergourd Momordica charantia. Indian J Exp Biol19:766-767. 27 WONG CM, YEUNG HW, NG TB, 1985 Screening of Trichosanthes kirilowii,Momordica charantia andCucurbita maxima (family Cucurbitaceae) for compounds with antilipolytic activity. J Ethnopharmacol 13(3):313-321. 28 YEUNG HW, LI WW, FENG Z, BARBIERI L, STIRPE F, 1988 Trichosanthin, alpha-momorcharin and beta-momorcharin: Identity of abortifacient and ribosome-inactivating protein. Int J Peptide Protein Res 31(3):265-268. 29 MIYAHARA Y, OKABE H, YAMAUCHI T, 1981 Studies on the constituents ofMomordica charantia L. II: Isolation and characterization of minor seed glycosides, momordicosides C, D and E. Chem Pharm Bull29(6):1561-1566. 30 SAUVAIN M, KODJOED JF, BERGRAVE SJ, BONNEVIE O, DEDET JP, 1986 Plantes fébrifuges en médecine traditionnelle en Haïti et en République Dominicaine et thérapie du paludisme. Rapport TRAMIL: ORSTOM, Cayenne, Guyane Française. 31 MORETTI C, 1989 Determinación de la actividad antimalárica de plantas utilizadas por la medicina tradicional (Momordica charantia). TRAMIL IV, Tela, Honduras, UNAH/enda-caribe. 32 CACERES A, JAUREGUI E, HERRERA D, LOGEMANN H, 1991 Plants used in Guatemala for the treatment of dermatomucosal infections. 1: Screening of 38 plant extracts for anticandidal activity. J Ethnopharmacol 33(3):277-283. 33 HEAL R, ROGERS E, WALLACE RT, STARNES O, 1950 A survey of plants for insecticidal activity. Lloydia13(2):89-162. 34 OGUNLANA EO, RAMSTAD E, 1975 Investigation into the antibacterial activities of local plants. Planta Med27(4):354-360. 35 HUSSAIN HSN, DEENI YY, 1991 Plants in Kano ethnomedicine; screening for antimicrobial activity and alkaloids. Int J Pharmacol29(1):51-56. 36 MANEELRT S, SATTHAMPONGSA A, 1978 Antimicrobial activity ofMomordica charantia. Undergraduate special project report. Bangkok, Thailand: Mahidol University. Faculty of Pharmacy. 37 LAL J, CHANDRA S, RAVIPRAKASH V, SABIR M, 1976 In vitro anthelmintic action of some indigenous medicinal plants on Ascardia galli worms. Indian J Physiol Pharmacol20(2):64-68. 38 BISWAS AR, RAMASWAMY S, BAPNA JS, 1991 Analgesic effect ofMomordica charantia seed extract in mice and rats. J Ethnopharmacol31(1):115-118. 39 MOKKHASMIT M, SAWASDIMONGKOL K, SATRAWAHA P, 1971 Study on toxicity of Thai medicinal plants. Bull Dept Med Sci 12(1/2):36-65. 40 GONZALEZ A, ALFONSO H, 1990 Evaluación de la toxicidad dérmica deMomordica charantia L.,Foeniculum vulgare Mill yCassia occidentalis L. en cobayos. Informe TRAMIL. Centro Nacional de Salud Animal, La Habana, Cuba. 41 GONZALEZ A, ALFONSO H, 1990 Evaluación de la toxicidad dérmica deMomordica charantia L. yCassia occidentalis L. en conejos. Informe TRAMIL. Centro Nacional de Salud Animal, La Habana, Cuba. 42 TABORA O, 1986 Estudio de toxicidad aguda en ratones de la fracción hipoglucemiante deMomordica charantia (Cucurbitaceae). Tegucigalpa, Honduras: IV Semana Científica Universidad Nacional Autónoma de Honduras UNAH. 43 SAKSENA SK, 1971 Study of antifertility activity of the leaves ofMomordica (karela). Indian J Physiol Pharmacol15(2):79-80. 44 PRAKASH AO, MATHUR R, 1976 Screening of Indian plants for antifertility activity. Indian J Exp Biol14(5):623-626. 45 SHARMA VN, SOGANI RK, ARORA RB, 1960 Some observations on hypoglycemic activity of Momordica charantia. Indian J Med Res48(4):471-477. 46 DIXIT VP, KHANNA P, BHARGAVA SK, 1978 Effects ofMomordica charantia fruit extract on the testicular function of dog. Planta Med34(3):280-286. 47 KOENTJORO-SOEHADI T, SANTA I, 1982 Perspectives of male contraception with regards to Indonesian traditional drugs. Bali, Indonesia: 2^nd National Congress of Indonesian Society of Andrology. 48 KHAN AH, BURNEY A, 1962 A preliminary study of the hypoglycemic properties of indigenous plants. Pakistan J Med Res2:100-116. 49 WEST M, SIDRAK G, STREET S, 1971 The anti-growth properties of extracts from Momordica charantia. West Indian Med J20(1):25-34. 50 NG T, 1988 Effects of momorcharins on ovarian response to gonadotropin induced superovulation in mice. Int J Fertil33(2):123-128. 51 DELAIGUE J, 2005 TRAMIL survey. UAG & PRDI, Tobago House of Assembly, Scarborough, Tobago.
	Petiveria alliacea (In territories with significant traditional TRAMIL use) Colombia : anamú Dominica : koujourouk Dominican Republic : anamú Guatemala : apacín Honduras : ipacina Haiti : ave Panama : anamú Significant uses found by the TRAMIL surveys leaf and stem, decoction, orally⁸	Recommandations Preparation and posology References	According to published and other information: Use for digestive conditions (stomach pain, bad or slow digestion and intestinal gas) toothache, muscle pain, skin diseases, rheumatism and common cold is classified as REC, based on the significant traditional use documented in the TRAMIL surveys and toxicity studies. Should there be a notable worsening of the patient’s condition, or should stomach pain persist for more than 3 days, seek medical attention. Use for headache, flatulence, flu and nasal congestion (sinusitis) is classified as REC, based on the significant traditional use (OMS/WHO)¹¹ documented in the TRAMIL surveys. For topical application, strict hygiene measures should be observed in order to avoid contamination or additional infection. Considering the risks of documented interactions with insulin or oral hypoglycemiants, the decoction of the leaf and stem should not be ingested by patients taking or using these medicines due to the risk of magnifying their effects. The root and the stem can cause reactions of hypersensitivity. Not for use during lactation or by children under 12 years old. Not for use during pregnancy because it may be abortifacient.		For digestive conditions and common cold: Prepare a decoction with 30 grams (3 spoonfuls) of ground leaf in 1 liter of water (4 cups). Boil for at least 10 minutes in a covered pot. Filter, allow to cool and drink 2-3 cups a day³⁷. For rheumatism: Prepare a decoction with 30 grams (3 spoonfuls) of ground leaf and root in 1 liter of water (4 cups) for at least 10 minutes in a covered pot. Filter, leave to cool down and drink 2-3 cups a day³⁷. For nasal congestion (sinusitis): Prepare a fine powder of the root and stem from dried and sieved material, inhale 0.2 to 0.5 grams through each nostril, 2 times a day³⁸. For headache, toothache, muscle pain, flatulence, flu and skin diseases: There is no available information establishing a means of preparation and dosage other than that referred to by traditional use. Any medicinal preparation must be preserved cold and used within the 24 hours.	1 GIRON L, 1988 Encuesta TRAMIL (Costa atlántica). Centro Mesoamericano de Tecnología CEMAT, Guatemala, Guatemala. 2 WENIGER B, ROUZIER M, 1986 Enquête TRAMIL. Service Oecuménique d'Entraide SOE, Port au Prince, Haïti. 3 WENIGER B, 1987-88 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 4 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (región Este). Dep. de Botánica, Jardín Botánico Nacional, Santo Domingo, Rep. Dominicana. 5 CHARLES C, 1988 TRAMIL survey. Movement for Cultural Awareness MCA, Roseau, Dominica. 6 LAGOS-WITTE S, Tinoco R, Merlo V, 1996 Encuesta complementaria TRAMIL. Laboratorio de Histología Vegetal y Etnobotánica, Dep. de Biología, Universidad Nacional Autónoma de Honduras UNAH, Tegucigalpa, Honduras. 7 GOMEZ H, GAITAN R, DIAZ F, 2003 Encuesta TRAMIL (Norte del departamento de Bolívar). Grupo de Productos Naturales, Facultad de Ciencias Químicas y Farmacéuticas. Universidad de Cartagena, Cartagena de Indias, Colombia. 8 SOLIS P, CORREA M, GUPTA M, 1995 Encuesta TRAMIL (Comunidades afro-caribeñas). Centro de Investigaciones Farmacognósticas de la Flora Panameña CIFLORPAN, Facultad de Farmacia, Universidad de Panamá, Panamá, Panamá. 9 GERMOSEN-ROBINEAU L, GERONIMO M, AMPARO C, 1984 Encuesta TRAMIL. enda-caribe, Santo Domingo, Rep. Dominicana. 10 Castillo D, Rodriguez S, de los Santos C, Belen A, 2003 Encuesta TRAMIL (Zambrana, Cotuí). Dep. de Botánica, Jardin Botánico Nacional, Santo Domingo, Rep. Dominicana. 11 WHO, 1991 Pautas para la evaluación de medicamentos herbarios WHO/TRM/91.4 (original inglés). Programa de Medicina Tradicional, OMS, Ginebra, Suiza. 12 WENIGER B, SAVARY H, DAGUIHL R, 1984 Tri phytochimique de plantes de la liste TRAMIL. Laboratoire de chimie des substances naturelles, Faculté de Médecine et de Pharmacie, Université d'Etat d'Haïti, Port au Prince, Haïti. 13 HEGNAUER R, 1973 Chemotaxonomy der Pflanzen. Basel, Schweiz: Birkhauser Verlag. 6:882. 14 SEGELMAN F, SEGELMAN A, 1975 Constituents of Petiveria alliacea. Lloydia 38(6):537. 15 DE SOUSA JR, DEMUNER AJ, PINHEIRO JA, BREITMAIER E, CASSELS BK, 1990 Dibenzyl trisulphide and trans-N-methyl-4-methoxyproline fromPetiveria alliacea. Phytochemistry 29(11):3653-3655. 16 FURONES JA, MORON F, PINEDO Z, 1996 Ausencia de la acción analgésica de la Petiveria alliacea (anamu) en ratones. Rev Cubana Planta Med 1(1):16-18. 17 DEL CARMEN RIVAS C, JIMENEZ M, AYALA L, CARILLO C, CABRERA Y, 1988 Actividad anti-inflamatoria y analgésica dePetiveria alliaceae. Informe TRAMIL. Centro de Investigación y Desarrollo de Medicamentos (CIDEM), La Habana, Cuba. 18 FURONES JA, MORON F, PINEDO Z, 1996 Ausencia de actividad antiinflamatoria del extracto acuoso liofilizado de Petiveria alliacea (anamú) en ratas. Informe TRAMIL. Rev Cubana Planta Med 1(2):34-37. 19 Martinez MJ, Betancourt J, Lopez M, MorejOn Z, Fuentes V, MORON F, PINEDO Z, Boucourt E, 2001 Actividad antimicrobiana y sobre varias preparaciones de músculo liso, in vitro, de la decocción liofilizada de hoja de Petiveria alliacea.Informe TRAMIL. Laboratorio Central de Farmacología, Facultad de Ciencias Médicas “Dr. Salvador Allende”, La Habana, Cuba. 20 GarcIa GM, Coto MT, GonzAlez CS, Pazos L, 1995 Velocidad del tránsito intestinal en ratones, del extracto acuoso de hoja fresca de Petiveria alliacea. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBI, Escuela de Medicina, Universidad de Costa Rica, San Pedro, Costa Rica. 21 ECHEVARRIA A, TORRES D, 2001 Efecto de un extracto de Petiveria alliacea Lin sobre el crecimiento de Giardia lamblia in vitro. Rev Cubana Med Mil 30(3):161-165. 22 CACERES A, GIRON LM, ALVARADO SR, TORRES MF, 1987 Screening of antimicrobial activity of plants popularly used in Guatemala for the treatment of dermatomucosal diseases. J Ethnopharm20(3):223-237. 23 CACERES A, LOPEZ BR, GIRON MA, LOGEMANN H, 1991 Plants used in Guatemala for the treatment of dermatophytic infections. 1. Screening for the antimicotic activity of 44 plant extracts. J Ethnopharm 31(3):263-276. 24 SAUVAIN M, 1989 Etude de plantes antiparasitaires du plateau des Guyanes en Amazonie: antipaludiques et antileishmaniens (Thèse de Doctorat). Université Paris-Sud, Paris, France. 25 RUFFA MJ, PERUSINA M, ALFONSO V, WAGNER ML, SURIANO M, VICENTE C, CAMPOS R, CAVALLARO L, 2002 Antiviral activity of Petiveria alliacea against the bovine viral diarrhea virus. Chemotherapy 48(3):144-147. 26 CACERES A, JAUREGUI E, HERRERA D, LOGEMANN H, 1991 Plants used in Guatemala for the treatment of dermatomucosal infections. 1: Screening of 38 plant extracts for anticandidal activity. J Ethnopharm 33(3):277-283. 27 LORES RI, PUJOL MC, 1990 Petiveria alliacea L. (anamu). Study of the hypoglycemic effect. Med Interne 28(4):347-352. 28 GERMANO DH, CALDEIRA TT, MAZELLA AA, SERTIE JA, BACCHI EM, 1993 Topical anti-inflammatory activity and toxicity of Petiveria alliacea. Fitoterapia 64(5):459-467. 29 FERRAZ MB, PEREIRA RB, IWATA NM, ATRA E, 1991 Tipi. A popular analgesic tea. A double blind cross-over trial in osteoarthritis. Clin Exp Rheumatol 9(2):205-206. 30 LIMA TCM, MORATO GS, TAKAHASHI RN, 1991 Evaluation of antinociceptive effect of Petiveria alliacea (guiné) in animals. Mem Inst Oswaldo Cruz 86(suppl.2):153-158. 31 GarcIa GM, Coto MT, GonzAlez CS, Pazos L, 1996 Toxicidad sub-crónica en ratones, del extracto acuoso de hojas frescas de Petiveria alliacea. Informe TRAMIL. Laboratorio de Ensayos Biológicos LEBI, Universidad de Costa Rica, San Pedro, Costa Rica. 32 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, MORON F, 2001 Toxicidad aguda (DL₅₀) oral de la decocción de hojas jóvenes frescas de Petiveria alliaceaL.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende. Ciudad de La Habana, Cuba. 33 GUERRA MO, OLIVEIRA AB, MAIA JGS, PETERS VM, 1989 Alteraçäo do desenvolvimento embrionário de ratos após tratamento com extratos aquosos de diferentes orgäos de Petiveria alliacea. Bol Centro Biol Reprod 8:17-22. 34 GERMANO DHP, SERTIE JAA, BACCHI EM, 1995 Pharmacological assay of Petiveria alliacea. II. Oral anti-inflammatory activity and gastrotoxicity of a hydroalcoholic root extract. Fitoterapia 66(3):195-202. 35 REYNOLDS J Ed., 1996 Martindale: The extra pharmacopoeia. Evaluated information on the world’s drugs and medicines. 31^st ed. London, England: The Royal Pharmaceutical Society. p1678. 36 BUDAVARI S Ed., 2001 The Merck Index: an encyclopedia of chemical, drugs, and biologicals. 30^th ed. Whitehouse Station, USA: Merck & Co., Inc. p181. 37 ALBORNOZ A, 1993 Medicina tradicional herbaria. Caracas, Venezuela: Editorial Instituto Farmacoterápico Latino S.A. p298. 38 CARBALLO A, 1995 Cálculo de concentración y dosis de las drogas vegetales TRAMIL: Mensuraciones farmacognósticas y aproximaciones técnico-clínicas. Laboratorio provincial de producción de medicamentos, Sancti Spiritus, Cuba. 39 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, 2001 Genotoxicidad in vivo: ensayo de morfología de la cabeza del espermatozoide en ratones de decocción liofilizada de hojas frescas de Petiveria alliacea L.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende, Ciudad de La Habana, Cuba. 40 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V. 2001 Genotoxicidad in vivo: ensayo de micronúcleos en médula ósea de decocción liofilizada de hoja fresca de Petiveria alliacea L. Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende, Ciudad de La Habana, Cuba. 41 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, 2001 Genotoxicidad in vitro: mediante el sistema de ensayo con Aspergillus nidulans de decocción liofilizada de hoja fresca Petiveria alliacea L. Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende, Ciudad de La Habana, Cuba. 42 GUERRA MJ, BETANCOURT J, LOPEZ M, MOREJON Z, BOUCOURT E, FUENTES V, 2001 Toxicidad aguda (DL₅₀) intraperitoneal de la decocción liofilizada de hojas frescas de Petiveria alliaceaL.Informe TRAMIL. Laboratorio Central de Farmacología. Facultad de Medicina Dr. Salvador Allende. Ciudad de La Habana, Cuba. 43 CACERES A, LOPEZ B, GONZALEZ S, BERGER I, TADA I, MAKI J, 1998 Plants used in Guatemala for the treatment of protozoal infections. I. Screening of activity to bacteria, fungi and American trypanosomes of 13 native plants. J of Ethnopharmacology 62(3):195-202.